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Automated cardiac sarcomere analysis from second harmonic generation images.

TitleAutomated cardiac sarcomere analysis from second harmonic generation images.
Publication TypeJournal Article
Year of Publication2014
AuthorsGarcia-Canadilla, P, Gonzalez-Tendero, A, Iruretagoyena, I, Crispi, F, Torre, I, Amat-Roldan, I, Bijnens, BH, Gratacos, E
JournalJ Biomed Opt
Volume19
Issue5
Pagination56010
Date Published2014 May 1
ISSN1560-2281
Abstract

<p>ABSTRACT. Automatic quantification of cardiac muscle properties in tissue sections might provide important information related to different types of diseases. Second harmonic generation (SHG) imaging provides a stain-free microscopy approach to image cardiac fibers that, combined with our methodology of the automated measurement of the ultrastructure of muscle fibers, computes a reliable set of quantitative image features (sarcomere length, A-band length, thick-thin interaction length, and fiber orientation). We evaluated the performance of our methodology in computer-generated muscle fibers modeling some artifacts that are present during the image acquisition. Then, we also evaluated it by comparing it to manual measurements in SHG images from cardiac tissue of fetal and adult rabbits. The results showed a good performance of our methodology at high signal-to-noise ratio of 20&nbsp;dB. We conclude that our automated measurements enable reliable characterization of cardiac fiber tissues to systematically study cardiac tissue in a wide range of conditions.</p>

DOI10.1117/1.JBO.19.5.056010
Alternate JournalJ Biomed Opt
PubMed ID24853145